The allantoic fluids from each passage were negatively stained with 2% sodium phosphotungstate and observed by electron microscopy, and haemagglutination activity of viruses in the allantoic fluids were examined by haemagglutination assay. 每代尿囊液经2%的磷钨酸钠负染色后在电镜下观察病毒粒子的形态特征,并用红细胞凝集的方法检测尿囊液中病毒的血凝活性。